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(54) on Spanish obese children aged 3 to 17 years, strong positive associations were found between some pro-inflammatory markers, such as monocyte chemoattractant protein-1 (MCP-1) and tumour necrosis factor-alpha (TNF-) and plasma total antioxidant capacity (TAC), in parallel with a significant positive association between OxS biomarkers and MCP-1

Primary and secondary antibodies were diluted in blocking solution (0.3% (v/v) Triton X-100, 10% (v/v) FBS in PBS)

Once the rats were killed, the colon was removed aseptically and placed on an ice-cold plate, longitudinally opened and the luminal contents were collected for the measurements of faecal moisture, pH and microbiological and SCFA production studies (see later)

Healthcare providers most commonly recommend IV therapy during pregnancy for specific medical situations where the benefits outweigh any potential risks

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